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Human monoclonal antibodies that bind insulin-like growth factor (IGF). See Harlow & Lane, Antibodies, A Laboratory Manual. 1, 200), 921-927, Malin Johansson, Eva K. Try the new Google Patents. FcRn, which is responsible for the transfer of. Patent US7. 96. 82. Device and method for high- throughput quantification of m. RNA from whole blood. RELATED APPLICATIONSThis application is a continuation of U. S. 1. 0/7. 96,2. 98 filed on Mar. B2, which is a continuation- in- part of U. S. 1. 0/6. 98,9. 67, filed on Oct. International Application No. PCTUS0. 3/1. 28. 95, which was filed in English on Apr. English, and claims the benefit of U. S. Provisional Application No. Apr. 2. 4, 2. 00. BACKGROUND OF THE INVENTION1. Field of the Invention. The present invention relates to high- throughput isolation and quantification of m. RNA from whole blood. Hakko FX-888D Soldering Station $65 Frys B&M . Today's Posts; Mark Forums Read; FAQ; Welcome to the The Garage Journal Board forums. Shop our selection of Pliers in the Tools. The unique tooth design allows for better grip and increases torque transfer with. More particularly, this invention relates to a method and device for isolating and amplifying m. RNA using combinations of leukocyte filters attached to oligo(d. T)- immobilized multi- well plates. Description of the Related Art. Research in the field of molecular biology has revealed that the genetic origin and functional activity of a cell can be deduced from the study of its ribonucleic acid (RNA). This information may be of use in clinical practice, to diagnose infections, to detect the presence of cells expressing oncogenes, to detect familial disorders, to monitor the state of host defense mechanisms and to determine the HLA type or other marker of identity. RNA exists in three functionally different forms: ribosomal RNA (r. RNA), transfer RNA (t. RNA) and messenger RNA (m. RNA). Whereas stable r. RNA and t. RNA are involved in catalytic processes in translation, m. RNA molecules carry genetic information. Only about 1- 5% of the total RNA consists of m. RNA, about 1. 5% of t. RNA and about 8. 0% of r. RNA. m. RNA is an important diagnostic tool, particularly when it is used to quantitatively observe up- or down- regulation of genes. Human peripheral blood is an excellent clinical resource for m. RNA analysis. The detection of specific chimeric m. RNA in blood, for example, indicates the presence of abnormal cells and is used in molecular diagnostics for chronic myelogenous leukemia (CML) (Kawasaki E. D., Champlin R., Witte O. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia- specific m. RNA sequences amplified in vitro. USA 8. 5: 5. 69. 8- 5. Pachmann K., Zhao S., Schenk T., Kantarjian H., El- Naggar A. Expression of bcr- able m. RNA individual chronic myelogenous leukaemia cells as determined by in situ amplification. Micrometastatic cancer cells can also be detected in blood by measuring cancer- specific m. RNA, such as carcinoembryonic antigen (CEA) for colon cancer, prostate specific antigen (PSA) for prostate cancer, thyroglobulin for thyroid cancer (Wingo S. Y., Solomon B., Nicholson D., Balducci- Silano P. Quantitative reverse transcription- PCR measurement of thyroglobulin m. RNA in peripheral blood of healthy subjects. Pelkey T. Molecular and immunological detection of circulating tumor cells and micrometastasis from solid tumors. Moreover, as the levels of these cancer- specific m. RNA can change following treatment, quantification of specific m. RNA provides for a useful indicator during treatment follow- up. As blood contains large quantities of non- nucleated erythrocytes (approximately 5 million cells/. However, due to inconsistencies in the recovery of specific subsets of leukocytes among different samples, the number of isolated leukocytes is determined for each sample and results are expressed as the quantity of m. RNA per leukocytes, not m. RNA/. Moreover, m. RNA quantities may change during lengthy isolation processes. While no method exists for the isolation of cancer cells from blood, gene amplification technologies enable the identification and quantification of specific m. RNA levels even from a pool of different genes, making whole blood an ideal material for m. RNA analysis when gene- specific primers and probes are available. The scientific community is facing a huge problem of institute- to- institute and experiment- to- experiment variation in gene expression analysis, because of the lack of standardization. Although recent gene amplification technologies provide an absolute quantity of template DNA, these values cannot be converted to the amounts of the gene in the original materials, due to the lack of information of the yield of RNA recovery and the efficiency of c. DNA synthesis in each sample. Total RNA is frequently used as a standardization marker for m. RNA quantitation, and results are typically expressed as the amounts of genes per . However, it must be emphasized that total RNA does not represent m. RNA, because the fraction of m. RNA is only 1- 5% of total RNA, and m. RNA volume varies even when the amounts of total RNA is identical. The yield of total RNA or m. RNA also varies widely depending on which method is employed. Once RNA is extracted, the next step is the synthesis of c. DNA, which itself can create uncertainty since existing methods do not indicate whether each RNA template creates a single copy of c. DNA in each experiment. In order to avoid the above problems, relative quantitation is used widely by comparing the data of target genes to that of housekeeping genes or r. RNA. However, the amounts of control genes are typically not consistent and may change during experiments. Moreover, this variation presents a serious problem for clinical diagnostics, since each clinical specimen is typically analyzed at a different point in time. It is typically very difficult to isolate pure m. RNA from whole blood because whole blood contains large amounts of RNAases (from granulocytes) and non- nucleated erythrocytes. Although various RNA extraction methods are available for whole blood applications (de Vries T. J., Fourkour A., Punt C. Analysis of melanoma cells in peripheral blood by reverse transcription- polymerase chain reaction for tyrosinase and MART- 1 after mononuclear cell collection with cell preparation tubes: a comparison with the whole blood guanidinium isothiocyanate RNA isolation method. Melanoma Research 1. Johansson M., Pisa E. K., Tormanen V., Arstrand K., and Kagedal Bl. Quantitative analysis of tyrosinase transcripts in blood. Y., Solomon B., Nicholson D., Balducci- Silano P. Quantitative reverse transcription- PCR measurement of thyroglobulin m. RNA in peripheral blood of healthy subjects. Consequently, there exists a need for a quick and easy method and device for isolating and quantifying large quantities of m. RNA from whole blood. Specifically, there exists a need for a high throughput, whole blood- derived m. RNA- processing technology with reproducible recovery and a seamless process to gene amplification. SUMMARY OF THE INVENTIONThe present invention discloses an efficient high throughput method and device for isolating and quantifying m. RNA directly from whole blood, with reproducible recovery, using combinations of leukocyte filters attached to oligo(d. T)- immobilized multi- well plates. One aspect of the invention includes a method of high throughput quantification of m. RNA in whole blood, including the steps of: (a) collecting whole blood; (b) removing erythrocytes and blood components from the whole blood by filtration to yield leukocytes on a filter membrane; (c) subjecting the leukocytes to cell lysis to produce a lysate containing m. RNA; (d) transferring the lysate to an oligo(d. T)- immobilized plate to capture the m. RNA; and (e) quantifying the m. RNA. In one preferred embodiment of the method, an anticoagulant is administered to the whole blood prior to collection of leukocytes. Several filter membranes can be layered together to increase the yield of captured leukocytes. The leukocytes that are trapped on the filter membrane are lysed using a lysis buffer to release m. RNA from the leukocytes. The transfer of lysate to the oligo(d. T)- immobilized plate can be accomplished using centrifugation, vacuum aspiration, positive pressure, or washing with ethanol followed by vacuum aspiration. The m. RNA is quantified by producing c. DNA and amplifying the c. DNA by PCR. Particularly preferred embodiments use Taq. Man PCR to quantify m. RNA. Another aspect of the invention comprises the use of artificial control RNA as a universal standard. In preferred embodiments, measuring the recovery of the standard RNA in each sample allows the results of gene amplification to used to determine the amounts of m. RNA present per . Embodiments of the present result in low coefficients of variation between samples and experiments. In preferred embodiments, variation can be minimized during RNA recovery, c. DNA synthesis, and quantification. The use of a standardized control RNA allows more efficient assays, quantification, and comparative testing. Another aspect of the invention includes a device for performing high throughput quantification of m. RNA in whole blood, wherein the device includes: (a) a multi- well plate containing: a plurality of sample- delivery wells; a leukocyte- capturing filter underneath the wells; and an m. RNA capture zone underneath the filter which contains immobilized oligo(d. T); and (b) a vacuum box adapted to receive the filter plate to create a seal between the plate and the box. In one preferred embodiment of the device, the leukocytes are captured on a plurality of filter membranes that are layered together. In another preferred embodiment of the device, the vacuum box is adapted to receive a source of vacuum. In another preferred embodiment of the device, a multi- well supporter is inserted between the vacuum box and the multi- well plates. Another aspect of the invention includes a kit, which contains: the device for performing high throughput quantification of m. RNA in whole blood, heparin, a hypotonic buffer, and a lysis buffer. Another aspect of the invention includes a fully automated system for performing high throughput quantification of m. RNA in whole blood, including: a robot to apply blood samples, hypotonic buffer, and lysis buffer to the device; an automated vacuum aspirator and centrifuge, and automated PCR machinery. BRIEF DESCRIPTION OF THE DRAWINGSFIG. Descargar El Quinto Acuerdo Miguel Ruiz Pdf Libro El Quinto Acuerdo Gratis. El jardin olvidado bajar en pdf el prisionero del cielo descargar libro pdf el nombre del viento. Zafon descargar . If you say so than to the Enterprise, said to think it out Cold War might very well have been decided against us. Bajo el Estigma del Quinto Sol. Facebook Email or Phone Password Forgot account? See more of Bajo el Estigma del Quinto Sol by logging into Facebook. 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